Hematology analyzer / blood cell counter function and working

The first method for counting cells is electrical impedance, also known as the Coulter principle.

Laser flow cytometry is more expensive than impedance analysis due to the need for expensive reagents, but gives detailed information about blood cells.

[{"selector":"#anim-09d5a58c-2ac9-4148-b771-0aaaa873d01e","keyframes":[{"transform":"scale(1)","offset":0},{"transform":"scale(1.3)","offset":0.33},{"transform":"scale(0.97)","offset":0.66},{"transform":"scale(1)","offset":1}],"delay":0,"duration":1450,"easing":"ease-in-out","fill":"both","iterations":1}] [{"selector":"#anim-a33902f4-6776-4acf-8abd-0df69b36963c","keyframes":[{"transform":"rotate(-540deg) scale(0.1)","opacity":0},{"transform":"none","opacity":1}],"delay":0,"duration":1000,"easing":"cubic-bezier(0.4, 0.4, 0.0, 1)","fill":"both","iterations":1}]

Adding fluorescent reagents extends the use of flow cytometry to measure specific cell populations.

[{"selector":"#anim-7d54b26b-69bf-4c38-ad62-fac5227d863b","keyframes":[{"offset":0,"transform":"translate3d(0, -177.02916%, 0)","easing":"cubic-bezier(.5, 0, 1, 1)"},{"offset":0.29,"transform":"translate3d(0, 0%, 0)","easing":"cubic-bezier(0, 0, .5, 1)"},{"offset":0.45,"transform":"translate3d(0, -49.780599792%, 0)","easing":"cubic-bezier(.5, 0, 1, 1)"},{"offset":0.61,"transform":"translate3d(0, 0%, 0)","easing":"cubic-bezier(0, 0, .5, 1)"},{"offset":0.71,"transform":"translate3d(0, -16.923987696%, 0)","easing":"cubic-bezier(.5, 0, 1, 1)"},{"offset":0.8,"transform":"translate3d(0, 0%, 0)","easing":"cubic-bezier(0, 0, .5, 1)"},{"offset":0.85,"transform":"translate3d(0, -6.355346844%, 0)","easing":"cubic-bezier(.5, 0, 1, 1)"},{"offset":0.92,"transform":"translate3d(0, 0%, 0)","easing":"cubic-bezier(0, 0, .5, 1)"},{"offset":0.96,"transform":"translate3d(0, -2.7616548959999996%, 0)","easing":"cubic-bezier(.5, 0, 1, 1)"},{"offset":1,"transform":"translate3d(0, 0%, 0)","easing":"cubic-bezier(0, 0, .5, 1)"}],"delay":0,"duration":1600,"fill":"both"}] [{"selector":"#anim-a223d6d7-6fba-4453-b11c-b72b4a55f093","keyframes":{"transform":["translate3d(105.68182%, 0px, 0)","translate3d(0px, 0px, 0)"]},"delay":0,"duration":600,"easing":"cubic-bezier(0.4, 0.4, 0.0, 1)","fill":"both"}] [{"selector":"#anim-1fb55dcf-183c-436b-8fe9-928dd32168d4","keyframes":{"opacity":[0,1]},"delay":0,"duration":600,"easing":"cubic-bezier(0.4, 0.4, 0.0, 1)","fill":"both"}] [{"selector":"#anim-3865fec5-1417-4421-a818-acc18aa721a9","keyframes":{"transform":["scale(0.15)","scale(1)"]},"delay":0,"duration":600,"easing":"cubic-bezier(0.4, 0.4, 0.0, 1)","fill":"forwards"}]

[{"selector":"#anim-ef076249-cb85-400f-a180-50c2f297d06a","keyframes":{"transform":["translate3d(115.18987%, 0px, 0)","translate3d(0px, 0px, 0)"]},"delay":0,"duration":600,"easing":"cubic-bezier(0.4, 0.4, 0.0, 1)","fill":"both"}] [{"selector":"#anim-62809e82-ed15-4cde-92ae-40a676302016","keyframes":{"opacity":[0,1]},"delay":0,"duration":600,"easing":"cubic-bezier(0.4, 0.4, 0.0, 1)","fill":"both"}] [{"selector":"#anim-b790c8ef-ec4e-4743-a4e6-5da8c4db8529","keyframes":{"transform":["scale(0.15)","scale(1)"]},"delay":0,"duration":600,"easing":"cubic-bezier(0.4, 0.4, 0.0, 1)","fill":"forwards"}] [{"selector":"#anim-693b89f1-17b5-4e4a-81b9-feeeb2ce04cc","keyframes":{"opacity":[0,1]},"delay":0,"duration":600,"easing":"cubic-bezier(0.2, 0.6, 0.0, 1)","fill":"both"}] [{"selector":"#anim-7799df14-f455-4fd9-ac0f-14d605ca59ec","keyframes":{"transform":["translate3d(-122.43589%, 0px, 0)","translate3d(0px, 0px, 0)"]},"delay":0,"duration":600,"easing":"cubic-bezier(0.2, 0.6, 0.0, 1)","fill":"both"}] Before 1956, manual counting of blood cells was done. After 1956, W. H. Coulter developed a system in which blood cells were counted using an electrical impedance procedure, also known as aperture impedance. And this was later known as coulter method. This innovation led to faster, greater access to blood counts

. In these instruments only RBCs, WBCs, hemoglobin, platelets and reticulocytes are specifically counted or measured. Hematocrit, red cell index and other values.

[{"selector":"#anim-c917a640-b2f5-44f0-8397-7cdf36fdb269","keyframes":[{"transform":"rotate(-540deg) scale(0.1)","opacity":0},{"transform":"none","opacity":1}],"delay":0,"duration":1000,"easing":"cubic-bezier(0.4, 0.4, 0.0, 1)","fill":"both","iterations":1}] [{"selector":"#anim-4c375b32-1aad-431b-a994-9be194d65664","keyframes":{"transform":["translate3d(-115.18988%, 0px, 0)","translate3d(0px, 0px, 0)"]},"delay":0,"duration":600,"easing":"cubic-bezier(0.4, 0.4, 0.0, 1)","fill":"both"}] [{"selector":"#anim-21dd8094-4563-4d06-83ef-8780b2fca6ee","keyframes":{"opacity":[0,1]},"delay":0,"duration":600,"easing":"cubic-bezier(0.4, 0.4, 0.0, 1)","fill":"both"}] [{"selector":"#anim-70ca7fd2-6093-4d6c-8a1b-786db14b4a53","keyframes":{"transform":["scale(0.15)","scale(1)"]},"delay":0,"duration":600,"easing":"cubic-bezier(0.4, 0.4, 0.0, 1)","fill":"forwards"}]

Hematology analyzer/ blood cell counter Function and Working Principle - Medical Technology (neutechmedical.com)

[{"selector":"#anim-91aec934-c9de-45c5-8f72-a06fc273b691","keyframes":{"opacity":[1,1]},"delay":0,"duration":2000,"easing":"cubic-bezier(.3,0,.55,1)","fill":"both"}] [{"selector":"#anim-11e8d2f3-a270-4c13-b44e-ae4a1ba0271a","keyframes":{"transform":["scale(3)","scale(1)"]},"delay":0,"duration":2000,"easing":"cubic-bezier(.3,0,.55,1)","fill":"forwards"}] [{"selector":"#anim-c236c5b7-2990-4574-9774-9f00cae599e4","keyframes":{"opacity":[0,1]},"delay":0,"duration":2000,"easing":"cubic-bezier(.3,0,.55,1)","fill":"both"}] [{"selector":"#anim-506925d5-c986-404a-ace1-f8b769d64d41","keyframes":{"transform":["scale(0.3333333333333333)","scale(1)"]},"delay":0,"duration":2000,"easing":"cubic-bezier(.3,0,.55,1)","fill":"forwards"}]